Work Package 6 - WP6

In NSTIs, different pathogenic mechanisms are likely to be involved causing cell death and widening of the intercellular spaces of basal cells and deeper tissues. Here, we explore mechanisms of pathogenesis and identify target molecules for novel therapeutic strategies using our unique skin tissue model.
The tissue model is exposed to live clinical bacterial isolates and isogenic bacterial mutants, bacterial supernatants with defined amounts of toxins, purified bacterial toxins (streptolysins/PVL/-hemolysin), as well as different therapeutic strategies including HBO and IVIG.
We (Partner 1) have previously applied such methodology to explore pathogenic actions of distinct S. aureus strains causing necrotizing pneumonia using a similar model system resembling human lung tissue. Histopathologic and phenotypic changes as well as cellular interactions are monitored by immunohistochemistry and immunofluorescence analyses. For these purposes light- and confocal- (Nikon A1R) microscopy are used. Data sets generated in this WP are correlated to patient data, disease manifestations and results obtained in WP1, 3 and 5. Similarly, correlations are made regarding the degradation of tissue structural proteins.

 

  • Mattias Svensson, PhD
    Leader for WP6
    Mattias Svensson, PhD
    Center for Infectious Medicine, Karolinska Institutet
    mattias.svensson@ki.se
    Karolinska University Hospital
    Karolinska University Hospital, Huddinge F59, S-141 86 Stockholm
    ki.se/cim/svensson
    Nikolai Siemens, Dr. rer. nat
    Center for Infectious Medicine, Karolinska Institutet
    nikolai.siemens@ki.se
    Karolinska University Hospital
    Karolinska University Hospital, Huddinge F59, S-141 86 Stockholm