This work package focus on the comprehensive and in-depth analysis of virulence gene repertoire of NSTI-causing pathogens, predominantly S. pyogenes and S. aureus.
Furthermore, we carry out transcriptomics, proteomics,and metabolomics analyses of selected sample sets from patients (WP2), artificial tissue (WP6), infected mice (WP1) and isolates from carriers or other disease eg. non-invasive pharyngitis and sepsis isolates (cohorts already available from partners 8 and 10). By assessing the virulence profile of isolates, we will provide new insights into the pathogen traits that affects disease outcome. Simultaneously, host samples are analyzed to decipher host components/pathways involved in outcome of infection. Combined with the clinical, phenotypic and functional data from WP1, 5, 6 and 7, this multiplex approach integrating various technologies will be pivotal for stratification of patients and biomarkers. These heterogeneous data sets will be integrated and analyzed through a series of multivariate statistics and reverse engineering procedures as described in WP4. Also, a data and model-driven experimental design jointly with WP4, assist in setting up testing experiments in mice (WP1) and tissues (WP6), and in selecting samples and measuring conditions.
- Leader of WP3
Professor Dietmar Pieper
Microbial Interactions and Processes
Helmholtz-Zentrum für Infektionsforschung GmbH
Inhoffenstraße 7, 38124 Braunschweig, GermanyAndreas Itzek, PhD
Department of Medical Microbiology
Helmholtz-Zentrum fuer Infektionsforschung GmbH
Inhoffenstraße 7, 38124 Braunschweig, GermanyProfessor Francois Vandenesch
Immunity Infection & Vaccination
Université Claude Bernard Lyon 1
INSERM Unit 1111, site Laennec, rue G. Paradin, 690008 Lyon